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Pharmacologia Vol. 8 (1), 2017
Research Article
Anti-Inflammatory Activity and Bioavailability Profile of Ethanolic Extract of Dichaetanthera africana
Aristide Laurel Mokale Kognou , Rosalie Annie Ngono Ngane , Rajesh Singh Pawar , Pradeep Pateriya , Prem Narayan Pal , Rebecca Madeleine Ebelle Etame , Alembert Tiabou Tchinda , Gabriel Agbor Agbor , Shweta Kumar , Raymond Simplice Mouokeu , Albert Sone Mouelle and Cecile Enguehard Gueiffier
Abstract: Background and Objective: Dichaetanthera africana (D. africana) is a traditional medicinal plant which is widely used in Cameroon, Gabon, Sierra Leone, Ivory Coast and Nigeria to treat various diseases such as coughs, chest ache and fatigue. This study aimed to evaluate anti-inflammatory activities and pharmacokinetic profile of the ethanolic extract of the stem bark of D. africana (EtOHDA). Materials and Methods: Ethanolic extract was evaluated for anti-inflammatory activities using in vitro and in vivo standard experimental models which included respectively protein denaturation, hypotonic-induced hemolysis and carrageenan paw edema assays. The characterization of the phytochemicals and bioavailability profiles of D. africana were investigated using high performance liquid chromatography-photodiode array detector (HPLC-PDA) analysis. The 16.0 SPSS windows software (SPSS Inc., Chicago, IL, USA) was used for the analysis of data. Results: The EtOHDA inhibited protein denaturation activity (3.12-82.59%) and protected the erythrocyte membrane against lysis induced by hypotonic solution (17.51-88.79%). Diclofenac sodium (78.22-94.53%) and indometacin (44.87-95.34%) showed the maximum inhibition. Significant reduction of paw edema (p<0.05) was observed in a dose-dependent manner after administration of the extract, especially at 400 mg kg–1 b.wt. These activities were comparable to the standard drugs while exhibiting no acute toxicity (LD50>2000 mg kg–1). Four major compounds were identified using HPLC, out of which only one was found in rat plasma. Conclusion: Results showed that EtOHDA has a significant anti-inflammatory activity. It is, therefore, a source of active compounds that might be used as anti-inflammatory agents.
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